Journal: Applied Optics

Article Title: Alignment and characterization of remote-refocusing systems

doi: 10.1364/AO.500281

Figure Lengend Snippet: Configurations of the transmitted-light and epi-fluorescence imaging modes of the test rig system. (a) Schematic of the transmitted-light mode. (b) Schematic of the epi-fluorescence imaging mode. (c) Top, exemplar z − x view of sub-regions of interest (sub-ROIs) of the same pinhole at different Stage 1 positions across the whole refocusing range acquired using the transmitted-light mode. Bottom, same for a single fluorescent bead acquired using the epi-fluorescence mode. Vertical gray lines separate the images taken at different Stage 1 positions. The white dashed vertical line indicates the position of the focal plane of O1. Scale bar, 5 µm; AL, aspheric lens; O, objective; TL, tube lens; M, mirror; L, lens; C, CMOS; D, doublet; F, filter; LD, Lambertian diffuser; STM, star test mask; CS, coverslip; DB, dichroic beamsplitter; PBS, polarizing beamsplitter; and QWP, quarter-wave plate. Red arrows in (a) correspond to the stage directions and component misalignment directions.

Article Snippet: In the transmitted-light mode, light from a 530 nm LED light source (M530F2, Thorlabs) is directed through an aspheric lens (AL, 350230-B, Thorlabs) with a focal length of 4.5 mm to create an illumination spot on a Lambertian diffuser (LD, 50 DO 50, Comar Optics).

Techniques: Fluorescence, Imaging

Journal: Sensors (Basel, Switzerland)

Article Title: A Tunable Hyperspectral Imager for Detection and Quantification of Marine Biofouling on Coated Surfaces

doi: 10.3390/s22187074

Figure Lengend Snippet: LED emission profiles. ( a ) Measured spectrum of the LED lamp driven at I = 1.5 A, which covers most of the visible wavelengths, thus overlapping with the LCTF transmission range. ( b ) Normalized irradiance at the output facet of the octagonal tapered hollow waveguide as simulated in ZEMAX ( 5 × 10 7 analysis rays, 1000 × 1000 pixels detector with 38 mm × 38 mm spatial dimensions, at 0 mm from taper output, smoothing = 2). The Lambertian LED profile was mixed through multiple internal reflections, generating a uniform irradiance pattern at the output facet.

Article Snippet: In this case, a calibrated Lambertian reflectance target with a spatially and spectrally homogeneous diffuse reflectance of R r e f ≈ 10 % was used (SphereOptics, RTI-010, 200 mm × 250 mm) that fills the full FOV.

Techniques: Transmission Assay

Journal: Sensors (Basel, Switzerland)

Article Title: HyScreen: A Ground-Based Imaging System for High-Resolution Red and Far-Red Solar-Induced Chlorophyll Fluorescence

doi: 10.3390/s22239443

Figure Lengend Snippet: Signal-to-noise ratio (SNR) and noise-equivalent-radiance (NER) of HyScreen’s FLUO module from 670–780 nm derived from Lambertian reference panels with reflectances of 5% (red), 20% (green), 50% (orange) and 95% (blue): ( a ) provides information on the SNR, and ( b ) shows the NERs of four Lambertian reference panels. The solid lines represent mean values of SNR or NER of across-track samples from regions of interest (ROIs), and the light-colored areas illustrate their corresponding standard deviations.

Article Snippet: Solar downwelling radiance of HyScreen is derived by measuring calibrated Lambertian diffuse reflectance reference panels made from Zenith Polymer ® (SphereOptics GmbH, Herrsching, Germany) placed at the beginning of the scanning area.

Techniques: Derivative Assay

Journal: Sensors (Basel, Switzerland)

Article Title: HyScreen: A Ground-Based Imaging System for High-Resolution Red and Far-Red Solar-Induced Chlorophyll Fluorescence

doi: 10.3390/s22239443

Figure Lengend Snippet: Signal-to-noise ratios (SNRs) and noise-equivalent-radiances (NERs) of HyScreen’s fluorescence sensor (FLUO) module from 670–780 nm derived from Lambertian reference panels with 5% and 20% reflectance: ( a ) provides information on the SNRs, and ( b ) shows the NERs of two Lambertian reference panels. The solid lines represent mean values of SNRs or NERs of across-track samples, which are from regions of interest (ROIs), and the light-colored areas illustrate their corresponding standard deviations.

Article Snippet: Solar downwelling radiance of HyScreen is derived by measuring calibrated Lambertian diffuse reflectance reference panels made from Zenith Polymer ® (SphereOptics GmbH, Herrsching, Germany) placed at the beginning of the scanning area.

Techniques: Fluorescence, Derivative Assay